Great Training Opportunities!
Educational Short Courses will be offered the Saturday and Sunday preceding the HPLC 2022 conference. The practical short courses will be taught by leading academic and industrial scientists covering both fundamentals as well as real-world application examples. The presentations will provide the valuable technical knowledge you need to implement techniques and solutions to improve job productivity, to solve today's separation problems as well as to understand tomorrow's technology. Short course notes will be emailed to each confirmed short course participant who should download and bring a copy of the notes with them. No printed copies will be available on site. HPLC 2022 reserves the right, without notice, to modify the short course material or schedules, as well as to amend the roster of short course presenters.
The cost to attend short courses is in addition to the symposium registration fee. Short courses are sold in half-day increments [each additional short course; limited availability; additional short courses available only to conference participants with a paid/purchased bundled, industry, academic, government or student conference meeting registration]. Full-day short courses equal two half-day short course purchases. For registrations paid at the Bundled rate, one free half-day short course is included. If a full-day short course is preferred, an additional half-day short course must be purchased. Short courses for NON-conference participants, and those without a paid/purchased Bundled meeting registration, are sold in half-day increments. Full-day short courses then require two half-day short course purchases.
|Short Course # - Full Day Saturday 9:00AM-4:00PM||Expert Instructors|
|1 - Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications||Dwight Stoll, Mark Schure|
|3 - Practical LC-MS/MS Method Development for the Practicing Scientists||Perry Wang|
|4 - Analysis and Characterization of Oligonucleotide Therapeutic Drugs||Claus Rentel|
|Short Course # - Half Day Sunday 9:00AM-12:00PM||Expert Instructors|
|5 - (u)HPLC Method Development (focusing on pharmaceutical analysis)||Michael Dong|
|7 - Field-Flow Fractionation (FFF): Polymer and Colloid Characterization||Jeff Ahlgren|
|8 - Chiral Liquid Chromatography||Michael Laemmerhofer|
|2 - Chromatography in the Analysis and Characterization of Protein Therapeutic Drugs||Koen Sandra|
|Short Course # - Half Day Sunday 1:00PM-4:00PM||Expert Instructors|
|9 - Capillary Electrophoresis with Mass Spectrometry Detection, More than just a Complementary Tool for LC-MS||David Chen|
|11 - 3D Printing and Microfluidics||Adam Woolley, Gregory Nordin|
|12 - HPLC Operation, Maintenance and Troubleshooting||Michael Dong|
|13 - Learning Chromatography in Chromatograms||Martin Gilar|
SHORT COURSE #1 (Full day Saturday 9:00am-4:00pm)
Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications
Instructiors: Dwight R. Stoll, Gustavus Adolphus College and Mark R. Schure, Kroungold Analytical, Inc.
In 2DLC, sample components are fractionated by two different columns utilizing different retention mechanisms. To achieve successful 2D resolution of complex sample components, dissimilar (orthogonal) retention mechanisms are required to effectively spread the peaks throughout the available separation space. In this course we will discuss in detail: • Theory of 2DLC, from a practical point of view • Instrumentation, including commercially available instruments • Method development across a wide variety of sample types • Applications to lipids, peptides, proteins, small and large molecule pharmaceuticals, biological extracts, industrial polymers, and surfactants • Helpful insights that are important for achieving good results in the lab Students are expected to be familiar with HPLC. Those who take this course will learn background information essential to understanding the technique and achieve practically useful results with commercial instrumentation. Many aspects of 2DLC are shared with one-dimensional HPLC such as column technologies, pumps, solvent systems, and matching the detector. However, 2DLC has some issues which are not present in one-dimensional HPLC, and these will be explained in detail so that course participants will have this knowledge prior to starting method development. We will also explore the suitable instrumentation for 2DLC, and how to process data external to the acquisition software. Applications of comprehensive 2DLC will be shown for complex industrial and biological samples, as well as simpler applications such as column switching, target peak purity investigation, and biopolymer analysis using commercial two-dimensional chromatographic instruments.
Mark Schure has worked in separation science for over 40 years in industry and academics. He is presently the Chief Technology Officer at Kroungold Analytical, a consulting firm he started in 2012. Dr. Schure is Adjunct Professor in the department of Chemical and Biomolecular Engineering at the University of Delaware; a position he has had for over 28 years and adjunct professor of chemistry at Drexel University. He has published over 120 papers, 4 patents and edited the book “Multidimensional Liquid Chromatography.” His scientific interests include the fundamental separation science of complex molecules, polymers and colloids, colloid chemistry, computational materials science and all aspects of solving large-scale chemical and physical problems with computers. He has received numerous awards including the Dal Nogare award, the Uwe D. Neue award and the Eastern Analytical Symposium award in separation science.
Dwight Stoll is Professor at Gustavus Adolphus College, where he teaches quantitative and instrumental analysis courses and is currently co-chair of the Chemistry Department. Active research projects in his laboratory touch upon most aspects of multi-dimensional separation methodologies, including optimization strategies, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis. Dwight is the author or co-author of more than 80 peer-reviewed publications and six book chapters in the area of separation science, has instructed numerous short courses in two-dimensional liquid chromatography, and has authored the LC Troubleshooting column for LCGC Magazine since 2017. In 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2014 he was named to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists. In 2017 he received the Georges Guiochon Faculty Fellowship, and was recognized with an Agilent Technologies Thought Leader Award, which is supporting research in his laboratory on the development of 2D-LC methodologies for biopharmaceutical analysis.
SHORT COURSE #2 (Half-day Sunday 9:00am-12:00pm)
Chromatography in the Analysis and Characterization of Protein Therapeutic Drugs
Instructor: Koen Sandra, CEO and Co-owner at RIC group and Visiting Professor, Ghent University
The characterization of therapeutic proteins such as monoclonal antibodies (mAbs) represents a tremendous challenge. This short course will highlight the possibilities offered by all the different modes of chromatography (i.e. RPLC, SEC, IEX, HIC, HILIC) and will focus on recent advances to rapidly and accurately characterize protein biopharmaceuticals (e.g., glycosylation, oxidation, deamidation, aggregation, lysine truncation…). The hyphenation of chromatographic approaches with mass spectrometry as well as the use of 2D-LC approaches will also be covered during the short course.
Koen Sandra received a PhD degree in Biochemistry from the Ghent University, Belgium in 2005. After his PhD, he joined Pronota, a molecular diagnostics company where he was active in developing analytical platforms for disease biomarker discovery and in setting up external collaborations. In 2008, he joined RIC, a company that provides chromatographic, electrophoretic and mass spectrometric support to the chemical, life sciences and pharmaceutical industries, where he holds the position of CEO. As a non-academic scientist, Koen Sandra is author of over 50 highly cited scientific papers and has presented his work at numerous conferences as an invited speaker.
SHORT COURSE #3 (Full day Saturday 9:00am-4:00pm)
Practical LC-MS/MS Method Development for The Practicing Scientists
Instructor: Perry G. Wang, Ph.D., LC-MS Technical Expert
This short course is designed to offer practical training for the practicing scientists in the analytical field. It will provide the participants with an updated overview and a solid working knowledge of LC-MS. The emphasis is on practical issues associated with LCMS method development and sample preparation. It also focuses on problem-solving skills with examples encountered in the analytical fields. It will take the participants stepby-step through the concepts and techniques to develop LC-MS methods. After this course, the participants will be able to independently develop their own LC-MS methods to use in their laboratories. New technologies and techniques, such as hydrophilic interaction liquid chromatography (HILIC), monolithic chromatography, and core-shell particles for HPLC will be presented.
WHO SHOULD ATTEND - This one-day course is intended for analytical chemists, researchers, supervisors, and lab managers, who use LC-MS. It will benefit all levels of professionals in the analytical field.
Perry G. Wang has been a chemist at US FDA since 2008. Prior to joining FDA, he worked in the pharmaceutical and medical-device industry for more than 10 years. He received his Ph.D. from Oregon State University. In addition to over 30 peer-reviewed publications, he has edited and co-edited five scientific reference books. He specializes in LC-MS method development and validation for drugs, cosmetics, foods, and dietary supplements. He teaches this course in his own capacity as a scientist, not as an employee of the FDA. He has been invited to teach this course at HPLC since 2012 and at the PittCon since 2007.
SHORT COURSE #4 (Full day Saturday 9:00am-4:00pm)
Analysis and Characterization of Oligonucleotide Therapeutic Drugs
Instructor: Claus Rentel, Vice President of Analytical Development Quality Control, Ionis Pharmaceuticals
This course will provide an overview of analytical techniques used for drug development of oligonucleotide therapeutics. Methods used for release and stability testing of recently approved antisense drugs will be presented including their development, validation and use in a quality control laboratory. Characterization tools employed to establish structure and properties of oligonucleotide drugs will be described, and approaches for the structural elucidation of oligonucleotide impurities covered. Expectations of regulatory agencies like FDA and EMA regarding testing and characterization of therapeutic oligonucleotides will be addressed.
Claus Rentel has more than 20 years of experience in quality control, analytical development and method validations. He has extensive expertise in the development of oligonucleotide therapeutics in regard to specifications, testing of starting materials and reagents, drug substance intermediates, drug substance, drug product and toxicological samples, as well as validation of IT quality systems. He has been responsible for the CMC drug substance section of IND filings for more than 40 oligonucleotide therapeutics, and participated in the NDA filings for KYNAMRO® (mipomersen), SPINRAZATM (nusinersen), WAYLIVRA® (volanesorsen), and TEGSEDITM (inotersen). Claus is currently Executive Director, Analytical Development and Quality Control at Ionis Pharmaceuticals, Inc., Carlsbad, California. Prior to joining Ionis in 2001 he worked in Quality Control and Special Analytics at CarboGen Laboratories AG, Aarau, Switzerland. He received his Ph.D. (summa cum laude) from the University of Tübingen, Germany.
SHORT COURSE #5 (Half-day Sunday 9:00am-12:00pm)
(u)HPLC Method Development (focusing on pharmaceutical analysis)
Instructor: Dr. Michael W. Dong, Norwalk CT, USA
This 3-hour course reviews best practices, short cuts, and tricks-of-the-trade to help pharmaceutical and other scientists to become more successful in developing effective HPLC or UHPLC methods (on potency and ICH-compliant stability-indicating assays of pharmaceuticals) using the traditional selectivity-tuning, 3-pronged method template, and universal generic gradient method approaches.
The Traditional 5-step Approach according to Snyder, Kirkland and Glajch
- Scouting gradient and getting the first chromatogram, method fine-tuning and optimization (Solvent strength/type, pH, buffer/additive, F, T, tG), automation screening systems, and software tools.
The 3-Pronged Template Approach for Rapid Method Development
- Fast LC isocratic methods for performance assessment, generic broad-gradient methods, and multi-segment gradient methods for ICH compliant stability-indicating assays of complex molecules; case studies.
A Universal Generic Gradient Method(s)
- A modernized generic method is capable of peak capacities of 100-300 in 2 to 6 minutes, rationales on the selection of column and operating conditions, method adjustments for stability-indicating applications, and case studies.
Michael W. Dong is a principal consultant in MWD Consulting. He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems/Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from the City University of New York and has 130+ publications, including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, 2019.)
SHORT COURSE #7 (Half-day Sunday 9:00am-12:00pm)
Field-Flow Fractionation (FFF): Polymer and Colloid Characterization
Instructor: Jeffrey A. Ahlgren, Senior Applications Scientist, Western Region, Wyatt Technology Corporation
One of the primary challenges for reliable and accurate characterization of biotherapeutics, nanoparticles, polymers, and proteins is effective analytical separation and detection. This is especially true for inherently polydisperse complexes, and those with aggregates or branched constituents. This workshop will explore the history, development and principles of operation, and latest advances in the area of flow Field-Flow Fractionation (FFF), coupled with multi-angle light scattering (MALS) to demonstrate how FFF — in a single-fluid pathway—enables highly tunable separations and robust sample characterization, while overcoming the inherent limitations of other techniques. The case studies presented will illustrate a broad suite of measurements afforded by FFF-MALS, including multi-detector analysis, molar mass determinations and high-resolution size distributions from 1–1000 nm, as well as quantitative particle concentration determination. Learn how these techniques are applied to a variety of biotherapeutics and functionally relevant polymers, proteins, and complexes. New capabilities presented will include sensitivity enhancements resulting from sample dilution control and advances in in-silico method development to shorten method development time.
Jeffrey Ahlgren is a Senior Applications Scientist for Wyatt Technology Corporation; he is currently based in San Diego, California. He came to Wyatt in 2002 after having been a Research Chemist for 13 years for the Agricultural Research Service, USDA, at the National Center for Agricultural Utilization Research in Peoria, Illinois. His research program at the ARS was focused on the characterization of polysaccharides from bacterial fermentation. He received his Ph.D. from the University of Illinois in Urbana-Champaign on the subject of freezing avoidance of Antarctic fishes, and post-doctoral research in the Department of Biochemistry at Duke University in Durham, North Carolina.
SHORT COURSE #8 (Half-day Sunday 9:00am-12:00pm)
Chiral Liquid Chromatography
Instructor: Prof. Michael Laemmerhofer, Eberhard-Karls-University of Tuebingen
In this course, after a short introduction to the basics of stereochemistry, the analytical and preparative scale separation of enantiomers and the currently available technologies for achieving this goal will be reviewed. After comparing different synthetic and separation strategies, the latter will be discussed in more detail with emphases on liquid phase techniques, such as high-performance liquid chromatography (HPLC) and super-/sub-critical fluid chromatography (SFC). All currently available modalities of liquid-phase separation techniques for analytical and preparative scale separation of enantiomers will be reviewed with focus on various chiral selectors and stationary phases and their performance from the viewpoint of analyte coverage, applicability in various separation mode(s) and their suitability for analytical and preparative purposes, respectively. The most widely used chiral selectors will be discussed, such as polysaccharide derivatives, along with the strategy of developing and selecting a promising CS, in combination with the optimal silica support. Modalities of attachment of a chiral selector to the silica surface will be also discussed. CSP preparation will be linked to the chromatographic performance of these materials. Significant attention will be paid to analytical and preparative method development with polysaccharide-based CSPs, enantiomer elution order, kinetics and thermodynamics of separation. Unusual observations will be discussed along with their significance for analytical and preparative-scale method development. Current trends in the field of enantioseparations, such as ultrafast separations, separation of chiral molecules with multiple chiral centers, etc. will be also reviewed . Reference:  B. Chankvetadze, Recent trends in preparation, investigation and application of polysaccharide-based chiral stationary phases for separation of enantiomers in high-performance liquid chromatography, Trends in Analytical Chemistry (TrAC), 122 (2020) 115709.
Michael Lämmerhofer is Full Professor (W3) for Pharmaceutical (Bio-)Analysis at the University of Tübingen, Germany (since 2011). He graduated in Pharmaceutical Sciences in 1992 and earned his Ph.D. in Pharmaceutical Chemistry in 1996 at the University of Graz, Austria (Supervisor: Prof. Wolfgang Lindner). Between 1997 and 2011 he was assistant professor and since 2002 associate professor at the University of Vienna, Department of Analytical Chemistry. Between 1999-2000 he spent a year of research as post-doc at the Department of Chemistry of the University of California, Berkeley with Prof. Frantisek Šveč. Since 2007, he is associate editor of Journal of Separation Science. His research interests include the development of functionalized separation materials (chiral stationary phases, mixed-mode phases, chemo- and bioaffinity materials, nanoparticles, monoliths), metabolomics and lipidomics, pharmaceutical analysis (impurity profiling, enantioselective analytics), multidimensional separations and biopharmaceuticals analysis (peptides, oligonucleotides, proteins, plasmids).
SHORT COURSE #9 (Half-day Sunday 1:00pm-4:00pm)
Capillary Electrophoresis with Mass Spectrometry Detection, More than just a Complementary Tool for LC-MS
Instructor: David D. Y. Chen, Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
LC-MS is currently the most important analytical techniques for many applications in biomedical research and pharmaceutical industry. CE-MS, on the other hand, hasn’t seemed to live up to its potential and has only been successfully used by a relatively small number of scientists. The reality though, is not always as it seems. CE technology has quietly been used in the application processes of the majority of the new drugs approved by the US FDA in recent years. With the improvement in the interface technology between CE and MS, the sensitivity and robustness of have been significantly improved, and CE-MS technology is poised to become an increasingly more important tool to be used to push the frontiers of biomedical and pharmaceutical research. The application of CE-MS, including capillary isoelectric focusing directly coupled to mass spectrometry (cIEF-MS) for the analysis of monoclonal antibodies, peptides, and small molecules will be discussed. New development in protein conformer analysis, characterization of in-solution protein structural differences, protein aggregation, and proton activity analysis by hydrogen-deuterium exchange analysis in conjunction with CE based separation, will also be demonstrated.
David Da Yong Chen received his Ph.D. in Chemistry in 1993 from the University of Alberta, where he also received postdoctoral training in Chemistry and the Department of Medical Microbiology and Infectious Diseases. In July 1994, he joined the faculty of the Chemistry Department at the University of British Columbia, where he is currently a Full Professor in the Faculty of Science, as well as an Associate Member of the Department of Anesthesiology, Pharmacology & Therapeutics in the Faculty of Medicine. In December 2013, he was appointed Distinguished Guest Professor at the Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Nanjing Normal University. Dr. Chen’s research interests include investigation of principles of fluid migration and chemical separation, development of novel separation methods and purification systems, and coupling micro separation systems to mass spectrometry. Dr. Chen was given the 2002 Royal Society of Chemistry (RSC) Award in Analytical Separation Methods, and the 2003 Charles McDowell Award for Excellence in Research, a gold medal given to the most outstanding young scientist at UBC. For his contribution in analytical chemistry, Dr. Chen received the W. A. E. McBryde Medal and the Maxxam Award from the Canadian Society for Chemistry in 2008 and 2015, respectively.
SHORT COURSE #11 (Half-day Sunday 1:00pm-4:00pm)
3D Printing and Microfluidics
Instructors: Adam T. Woolley, Brigham Young University and Gregory P. Nordin, Brigham Young University
The workshop will introduce the technique of 3D printing, describe different types of 3D printers, and detail how these printers can be used to create microfluidic devices, providing a comparison to traditional microfluidic fabrication methods. Based on their extensive experience, the presenters will then discuss the current state-of-the art for component miniaturization and integration of 3D printed active elements such as channels, columns, valves, and pumps into more complex device designs. Attendees will leave with an understanding of advantages and limitations of 3D printing for microfluidic device fabrication, an appreciation of the challenges and opportunities in creating 3D printed microfluidic devices, and guidance on whether 3D printing microfluidics can advance their intended applications.
3D printing - fundamentals
Microfluidics - design and fabrication
Traditional fabrication methods
3D printing for microfluidics
3D printed components
Channels (including for microchip CE)
Higher level integration
Fluid and pneumatic I/O
Where to from here?
Adam T. Woolley earned his Ph.D. degree in Chemistry from the University of California, Berkeley in 1997, and was a Runyon-Winchell Postdoctoral Fellow at Harvard University from 1998-2000. He has been on the faculty in the Department of Chemistry and Biochemistry at Brigham Young University since 2000, where he is currently University Professor and Dean of Graduate Studies. Woolley’s research is at the interface between miniaturization and biomolecules; he is developing 3D printed microfluidics for bioanalysis, devising miniaturized assays for disease-linked biomarkers, and developing biotemplated fabrication of nanoelectronic devices.
Gregory P. Nordin received a Ph.D. in Electrical Engineering from the University of Southern California in 1992. From 1984 to 1992 he also worked at the Hughes Aircraft Company, the last three years of which were at the Hughes Research Laboratories in Malibu, California. From 1992 to 2005 he was in the Electrical and Computer Engineering Department at the University of Alabama in Huntsville where he was founding director of the university's Nano and Micro Devices Center. In 2005 Dr. Nordin joined Brigham Young University as Professor in the Electrical and Computer Engineering Department. His research currently focuses on 3D printing for microfluidics, and micro- and nano-fabricated devices for biosensing, photonics, and MEMS.
SHORT COURSE #12 (Half-day Sunday 1:00pm-4:00pm)
HPLC Operation, Maintenance, and Troubleshooting
Instructor: Michael W. Dong, Norwalk CT, USA
This 3-hour short course provides the attendees with an overview of the best practices in HPLC/UHPLC operation, including mobile phase and sample preparation for pharmaceutical analysis. Common HPLC and UHPLC maintenance procedures are described together with HPLC troubleshooting strategies illustrated with practical case studies.
- Safety and Environmental concerns
- Mobile Phase and Sample Preparation
- Best Practice and standard operating procedure in HPLC Operation: Pumps, autosamplers, columns, UV detectors, and data systems.
HPLC Maintenance and Troubleshooting
- Common (u)HPLC maintenance procedures – what the lab analysts can do!
- Diagnosing and Solving Problems (pressure, baseline, peak, data performance) illustrated with case studies).
Dr. Michael W. Dong is a principal consultant in MWD Consulting. He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Senior Staff Scientist at Applied Biosystems/Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from the City University of New York and has 130+ publications, including a bestselling book on chromatography (HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, 2019
SHORT COURSE #13 (Half-day Sunday 1:00pm-4:00pm)
Learning Chromatography in Chromatograms
Instructor: Martin Gilar, Waters Corp., Milford, MA
The course attendees will learn about principles of chromatographic modes including reversed-phase, hydrophilic interaction, ion-exchange and size exclusion chromatography. Special emphasis will be given to reversed phase mode. Couse attendees will use MS Excel spreadsheets prepared to visualize chromatograms. Attendees will be able to change parameters such as particle size, column length, separation condition, extra column dispersion and others and instantly observe the effects in chromatographic separation. We will cover:
- Principles of chromatographic separation, isocratic retention versus gradient
- Retention, resolution, peak capacity, band broadening, extra column dispersion
- Chromatographic behavior of small molecules versus biopolymers
- Approaches to method development and method transfer.
- SPE versus LC
- Chromatographic artifacts of large volume injection, incompatible solvents, etc.
WHO SHOULD ATTEND: Chromatography practitioners, lab personal, students, casual LC users who are interested in deeper understanding of chromatographic principles. The attendees should bring their own laptop computers with installed MS Excel. The attendees will be provided with excel sheets based chromatographic simulators. They will be able to perform their own in-silico chromatographic experiments.
Martin Gilar received his Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he has worked at Waters Corp. in Milford, Massachusetts, participating in column, sample preparation and instrument research. Dr. Martin Gilar is a principal investigator in the Core Research group at Waters Corporation. He has more than 30 years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is the analysis of biopolymers, peptides and nucleic acids. He has published over 70 peer reviewed papers.