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List of Invited Speakers

JEFF AHLGREN, Senior Applications Scientist, Wyatt Technology Corporation, Santa Barbara, CA, USA

Asymmetrical Flow Field Flow Fractionation with Multi-angle Light Scattering for characterizing Lipid Nanoparticles containing RNA

Bio: Jeffrey Ahlgren is a Senior Field Applications Scientist for Wyatt Technology Corporation’s Western Region, and is currently based in San Diego, California.  Prior to joining Wyatt Technology in 2002, he was a Research Chemist for the U.S Department of Agriculture’s Agricultural Research Service (ARS) at the National Center for Agricultural Utilization Research in Peoria, Illinois.  He received his Ph.D. from the University of Illinois Urbana-Champaign studying the antifreeze glycopeptides of Antarctic fish and was a Post-Doctoral Research Associate in the Department of Biochemistry at Duke University in Durham, North Carolina, studying enzymatic glycosylation of mucin proteins.

Alhgren Jeff

JARED ANDERSON, Alice Hudson Professor of Chemistry, Department of Chemistry, Iowa State University, Ames, Iowa USA

Determining the Loading of Oligonucleotides to Magnetic Liquid Supports for Sequence-specific Nucleic Acid Extraction using HPLC

Bio: Jared L. Anderson is the Alice Hudson Professor of Chemistry at Iowa State University and Faculty Scientist at Ames National Laboratory. Prior to joining Iowa State University, he was a Professor of Chemistry at The University of Toledo from 2005-2015 where held the ranks of assistant professor, associate professor, and full professor. His research focuses on the development of stationary phases for multidimensional chromatography, alternative approaches for sample preparation, particularly in nucleic acid isolation and purification, and analytical tools for trace-level analysis within active pharmaceutical ingredients. His research also explores the synthesis and use of green solvents in analytical separations as well as the use of smartphones in chemical analysis.  He has published over 200 peer-reviewed publications, 5 book chapters, and has co-edited a book series titled “Analytical Separation Science”. He currently holds 6 patents and is an editor for the Journal of Chromatography A.

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ERIN BAKER, Associate Professor, Department of Chemistry, North Carolina State University Raleigh, NC, USA

Combining Multidimensional Measurements with Standards and Isotopologue Workflows to Detect, Identify and Validate Molecules in Omic Studies

Bio: Erin S. Baker is an Associate Professor at North Carolina State University in Raleigh, NC. To date, she has published over 140 peer-reviewed papers utilizing ion mobility spectrometry in conjunction with mass spectrometry (IMS-MS) to study both environmental and biological systems. Erin has served on the ASMS Board of Directors as the Member at Large for Education and is currently serving as the Vice President of Education for the International Lipidomics Society, Events Committee Chair for Females in Mass Spectrometry (FeMS) and as an Associate Director in the NCSU Comparative Medicine Institute. She is also an Associate Editor for the Journal of the American Society for Mass Spectrometry and on the Advisory Board for Journal of Proteome Research and Analytical and Bioanalytical Chemistry. She has received seven US patents, two R&D 100 Awards, been named to the Analytical Scientist 2019 and 2021 Top 100 Power List, aided in the commercialization of the Agilent 6560 IMS-QTOF MS, and was a recipient of the 2016 ACS Rising Star Award for Top Midcareer Women Chemists. Currently, her research group utilizes advanced separations, multi-omic analyses and novel software capabilities to examine connections between human health and the environment.

Baker, Erin

BLAIR BERGER, Post-doctoral fellow, University of Texas at Arlington, Arlington, TX, USA

Streamlined Stationary Phase Selection Facilitated by a ‘Sample-plug Retention Test’ in Supercritical Fluid Extraction-Supercritical Fluid Chromatography-Mass Spectroscopy (SFE-SFC-MS) Method Development for On-line Extraction of Anabolic Agents

Bio: Blair Berger, is a postdoctoral researcher at the University of Texas at Arlington (UTA).  She has a bachelor’s degree in marine-biology/chemistry, a master’s degree in natural products LCMS-based metabolomics and her doctorate in analytical chemistry. She has worked with SFC intermittently since 1998, in industry for companies such as Berger Instruments, Mettler-Toledo AutoChem, and later for Aurora SFC Systems during the commercial development phase of their SFC conversion module.  Working as an analytical chemist and customer support technician, she has co-published a number of peer-reviewed papers, technical notes, and user manuals.  While briefly working in academia (2017-2019), she also managed the Natural Products Laboratory liquid chromatography facility for the Institute of Biology at Leiden University in the Netherlands.  Recently receiving her PhD at UTA, her doctoral and postdoctoral research focuses on providing solutions for unique challenges associated with method development using hyphenated instrumentation (via SFE-SFC-MS) for online extraction/analysis of complex biological and environmental matrices.

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BI CONG, Principal Scientist, Chemical Process Development, Product Development, Bristol Myers Squibb, New Brunswick, NJ, USA

Development and Optimization of a Robust HPLC Method for Analytical Process Control of a Pharmaceutical Intermediate

Bio: Cong is a Principal Scientist in the department of Chemical Process Development at Bristol Myers Squibb. She received a PhD degree in Analytical Chemistry from the University of Nebraska-Lincoln in 2016. After her PhD, she joined Monsanto based in St. Louis, MO, working as an analytical chemist focused on the development of novel active ingredients for agrochemicals. Cong moved to New Jersey and joined BMS in 2018. Since then she has been responsible for supporting pharmaceutical process development for drug substances and intermediates, and has extensive experiences in the establishment of phase-appropriate analytical control strategies for drug substance scale-up process development and optimization, as well as systematic method development, validation, and technology transfer of analytical methods for active pharmaceutical ingredients, intermediates, and starting materials.

Cong, Bi

DEIRDRE CABOOTER, Department of Pharmaceutical and Pharmacological Sciences, University of Leuven, Leuven, BELGIUM

Possibilities of Machine Learning for Automation of Method Development in Liquid Chromatography

Deirdre Cabooter is a tenured research professor at the Department of Pharmaceutical and Pharmacological Sciences at the University of Leuven (KU Leuven, Belgium). She obtained a PhD in Chemical Engineering under supervision of Prof. Gert Desmet in 2009. Her current research is directed towards a deeper understanding of the fundamentals of liquid chromatography, the evaluation of novel supports in chromatography, the analysis of complex samples in pharmaceutical, bio-analytical and environmental applications, retention modeling and solutions for automated method development. She has published over 100 peer-reviewed papers and book chapters, and is currently the promoter of 10 PhD students. She received the LCGC Emerging Leader in Chromatography Award in 2017, the Jubilee Medal of the Chromatographic Society in 2020, and is an editor of Journal of Chromatography A since 2018.

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MARTINA CATANI, Assistant Professor, Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, ITALY

Process Intensification in the Biopharma Industry: Improving Purification of Therapeutic Peptides through Multicolumn Continuous Countercurrent Preparative Liquid Chromatography

Bio: Martina Catani is assistant professor in analytical chemistry at the University of Ferrara (Dept. of Chemical, Pharmaceutical and Agricultural Sciences) since July 2021 and she received a PhD in chemical sciences in 2018 from the same university. She has spent research periods at VUB Brussels (Belgium), University of Pécs (Hungary) and ETH Zurich (Switzerland) where she worked as a post-doctoral research fellow in 2019. Martina works in the field of liquid chromatography for both analytical and preparative purposes. Her main research activities are focused on purification of polypeptides, oligonucleotides, proteins by means of single-column and continuous countercurrent multicolumn preparative LC; separation of natural cannabinoids; investigation of kinetic and thermodynamic phenomena in chiral and achiral HPLC and SFC. She is co-author of more than 40 papers on peer reviewed journal and she has presented her research activities in more than 20 national and international scientific meetings. She has been the recipient of the international “Csaba Horváth Young Scientist Award” at HPLC2018 Washington (Washington, USA), of the “2020 Young Researcher Award” conferred by the Interdivisional Group of Separation Science of the Italian Chemical Society (SCI) and of the “2021 Young Researcher Award” conferred by the Analytical Chemistry Division of SCI.

Catani, M

WIM DE MALSCHEVrije Universiteit Brussel, Brussel, BELGIUM

Reduction of C-term Dispersion in AC EOF Vortex Chromatography

Bio: After obtaining his degree in Bio-engineering at the Vrije Universiteit Brussel (VUB) in 2004, Wim De Malsche moved to Mesa+ Institute for Nanotechnology (UTwente, NL) to develop prototypes of novel chip-based chromatography systems and to obtain a PhD in Applied Physics. He returned to Belgium in 2008 to valorize his results and (co-)founded the spin-off company PharmaFluidics in 2010, where he was also subsequently (part-time) CEO and CTO and (currently) director. From 2009-2015 he was FWO research fellow allowing him to conduct his research at the Interuniversity Micro-Electronics Center (Imec, Leuven), VUB and UTwente. He is Faculty member at VUB since 2012, where he teaches Environmental Technology and Nanotechnology. Since 2016 he teaches Microfluidics for Lab-on-a Chip at EPFL (Lausanne, CH). In 2012 he started his µFlow group (currently 10 researchers) that is designing, developing, fabricating and validating novel microfluidic devices for a wide range of applications involving e.g. analytical and continuous separations, particle and droplet production, handling viscous and challenging streams and advanced optics. At VUB he is coordinating a Strategic Research Program on the development of microfluidic and optical techniques for a wide range of biological, medical and (bio)chemical applications. In 2016 he obtained an ERC starting grant to enhance mass transfer in microfluidic devices.

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JOHN DOLAN, LC Resources, McMinnville, OR, USA

Recipient of the 2022 Uwe D. Neue Award in Separation Science

In the Fullness of Time: The DryLab® Story

Bio: After finishing graduate school (UC Davis) and a postdoc, John Dolan started LC Resources with Lloyd Snyder (later joined by Tom Jupille) in 1984 and worked there until his retirement in 2017. His work involved managing the LC Resources laboratory, teaching professional training courses, doing R & D, and, for 34 years, writing the monthly “LC Troubleshooting” column in LCGC. The LCR laboratory provided HPLC-oriented services for the pharmaceutical industry as well as a resource to support LCR’s in-house research. Over the years, John taught more than 10,000 students LC- and LC/MS-related topics ranging from introductory material to advanced method development and troubleshooting. His research concentrated on two main areas – systematic HPLC method development using computer-aided techniques and the development of the Hydrophobic-Subtraction Model (HSM) of reversed-phase column selectivity. These resulted in more than 150 publications (mostly co-authored with Lloyd Snyder), the DryLab® method development software (now part of the Molnar Institute), and an HSM database comparing selectivity of nearly 800 reversed-phase columns (now administered by Dwight Stoll). Although now retired from teaching LC classes, John and Tom Jupille recorded all their LC training classes to make them available as on-demand web-based learning modules (administered by Analytical Training Solutions).

Dolan, J

ANDREAS DUNKEL, Leibniz-Institut für Lebensmittel-Systembiologie an der Technischen Universität München (LSB), GERMANY

Metabolomics Analysis of Complex Food Systems to Improve Sensory Quality and Sustainability
DUNKEL Andreas at Leibniz-Institut

SEBASTIAAN EELTINK, Full Professor, Department of Chemical Engineering, Vrije Universiteit Brussel, BELGIUM

Novel Microfluidic Solution for Establishing the Next Generation of Two- and Three-dimensional Liquid Chromatography

Bio: Sebastiaan Eeltink received his PhD degree in Chemistry (specialization Analytical Chemistry) in 2005 from the University of Amsterdam (The Netherlands) for his dissertation “Packed and monolithic capillary columns for LC”. From 2005-2007 he conducted post-doctoral research at the University of California, Berkeley, USA, and was guest scientist at the Lawrence Berkeley National Laboratory. Here he developed novel column formats, including coated capillary columns and monolithic structures in microfluidic chips. In 2007 he joined Dionex (currently Thermo Fisher Scientific) and conducted research on packed and monolith column technology for ultra-high-pressure LC, two-dimensional LC, and nano LC. In 2009 the National Fund for Scientific Research (FWO, Belgium) awarded him an Odysseus grant and currently he holds a position as research professor at the Department of Chemical Engineering at the Free University of Brussels. He teaches courses in the field of separation sciences and is first or senior author of more than 70 scientific publications and 3 patent applications on spatial three-dimensional liquid chromatography.

EELTINK Sebastiaan

ATTILA FELINGER, Professor, University of Pecs, Pecs, HUNGARY

Solvent Interactions in Supercritical Fluid Chromatography

Bio: Attila Felinger is a Professor of Analytical Chemistry at the University of Pécs (Hungary), in addition to serving as President of the Hungarian Society for Separation Sciences. He graduated with a degree in chemical engineering from the University of Veszprém (Hungary) and obtained his Ph.D. in chemistry in 1988 from the same university; he also holds a D.Sc. degree from the Hungarian Academy of Sciences. His research interests focus on the fundamentals of chromatography including nonlinear, preparative, and analytical separations, as well as the statistical analysis of measurements by analytical chromatography. Prof. Felinger has published some 100 scientific papers and two books, and serves on the Editorial Board of Journal of Chromatography A and LC-GC Europe.

FELINGER Attila

FRANTISEK FORET, Director, Institute of Analytical Chemistry of the CAS, Brno, CZECH REPUBLIC

Preparative DNA Separation - Can Electrophoresis Bring Something New?

Bio: Frantisek Foret is the director of the Institute of Analytical Chemistry and head of the Department of Bioanalytical Instrumentation. He obtained his Ph.D. from the Institute of Analytical Chemistry in Brno, Czechoslovakia, in 1991 and DSc (Research Professor) in 2017 from the Czech Academy of Sciences. After a postdoctoral stay (1991) at the Barnett Institute, Northeastern University in Boston, he spent nine years there as a research group leader (1992 – 2001). His main research interests include capillary separations, laser-based detection, miniaturization, and mass spectrometry coupling. Besides working as a senior deputy editor of Electrophoresis, he is also an Associate Director of CASSS – an International Separation Science Society and member of the Learned Society of the Czech Republic. Dr. Foret has authored and co-authored over 200 publications listed by WoS, a monograph on Capillary Zone Electrophoresis, 15 book chapters, dozens of invited and plenary lectures at international conferences, and 22 patents. WoS citations (without auto citations) over 6000; h-index 47.

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TORGNY FORNSTEDT, Professor, Department of Engineering and Chemical Sciences, Karlstad University, Karlstad, Sweden

Analysis of Therapeutic Oligonucleotides using Ion-pair Chromatography: Current Understanding, Challenges and Machine-learning Building

Bio: Torgny Fornstedt obtained his M.Sc. in Pharmacy at Uppsala University 1986, obtained his PhD in Analytical Pharmaceutical Chemistry at Uppsala University 1992, since 2010 he is professor in Analytical Chemistry at Karlstad University, Sweden. He heads the Fundamental Separation Science Group (FSSG.se) that conduct theoretically advanced experimental research aiming at analysis, purification and characterization of drug molecules and high-value chemicals with special focus on industrial challenges. Recently, his research group and industrial partners focus in analysis and purification of therapeutic oligonucleotides and how to use modern digitalization technology for enhanced quality assurance of these next generation drugs.

Fornstedt, Tony

ANDREA GARGANO, Tenure Track Assistant Professor, University of Amsterdam, van’t Hoff Institute for Molecular Science (HIMS), Centre for Analytical Science Amsterdam, THE NETHERLANDS

Poly(acrylamide-co-N,N′-methylenebisacrylamide) Monoliths for Low Flow Liquid Chromatography - Mass Spectrometry of Analysis of Intact Proteins

Bio: Andrea Gargano is a tenure track assistant professor in Analytical Chemistry at the van’t Hoff Institute for Molecular Science, University of Amsterdam (NL). In his research, he focuses on developing LC-MS technology for the characterization of synthetic polymers and proteins. This includes developing hyphenated separation approaches for intact proteins analysis by native&denaturing top-down mass spectrometry, the use of new enzymatic systems to characterize synthetic polymers by mass spectrometry and instrumental development of multidimensional separation coupled with mass spectrometry. He received his Ph.D. in (Analytical) Chemistry from the University of Vienna (AU), did his post-doctoral fellowship at the University of Amsterdam (NL) and has been guest researcher in several research labs in the EU and USA (e.g. PNNL, Northwester University, LBNL). Andrea is an enthusiastic separation-science experimentalist and published research on the synthesis of stationary phases, chiral separations, two-dimensional LC separations and LC and MS methods for proteins and polymer analysis. He received the Ernst-Bayer Award in 2014, the Csaba Horváth Young-Scientist Award in 2015, and he was awarded an NWO-VENI research fellowship for his project on Intact-Protein Analysis (IPA). In 2018, the Analytical Scientist magazine listed me among the 'Fab Forty' of gifted young scientists 'making waves in analytical science.

Gargano, A

MARTIN GILARScientific Fellow, Waters Corporation, Milford, MA, USA

Analysis of New Drug Modalities: Oligonucleotides, siRNA, and mRNA

Bio: Dr. Martin Gilar (*1966) received his Ph.D. in analytical chemistry from Institute of Chemical Technology in Prague (1996). He spent postdoc years in Hybridon Inc. (1996-1998) and Northeastern University in Boston (1998) developing separation methods for antisense oligonucleotides and fraction collector for DNA molecules. Since 1998 he has worked at Waters Corp. in Milford, Massachusetts, participating in column, sample preparation and instrument research. Dr. Martin Gilar is a Scientific Fellow in the Separations R&D group at Waters Corporation. He has more than 30 years of experience in the separation sciences, including chromatography, electrophoresis, and mass spectrometry. His research interest is the analysis of biopolymers, peptides, and nucleic acids. He has published over 70 peer reviewed papers.

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JAMES GRINIAS, Assistant Professor, Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ, USA

Implementation of Compact Capillary LC into Analytical Workflows

Bio: James Grinias is Assistant Professor in the Department of Chemistry & Biochemistry at Rowan University in Glassboro, NJ. Dr. Grinias has a number of research interests focused on chemical separations and microfluidics, both at the fundamental level and for the analysis of biological systems. He was previously granted fellowships from the National Science Foundation for his graduate work at the University of North Carolina at Chapel Hill (where he was also a member of the Royster Society of Fellows) and from the National Institutes of Health for his postdoctoral research at the University of Michigan. Dr. Grinias’ graduate research on liquid chromatography also led to him being named a Csaba Horváth Young Scientist award winner at the 2013 HPLC Conference, a top award for young researchers in the field. At Rowan, he teaches courses in general, analytical, and bioanalytical chemistry while also conducting research on UHPLC column performance and instrument miniaturization.

Grinias

FABRICE GRITTI, Principal Consulting Scientist, Waters Corporation, USA

Advances in Chemistry-retention and Morphology-efficiency Relationships to Support Method Development in Liquid Chromatography

Bio: Fabrice G. Gritti received a B.S. degree in Chemistry and Physics from the University Joseph Fourier of Grenoble (France) in 1995, a Graduate Engineering School degree in Chemistry and Physics from the University of Bordeaux I (France) in 1997, and a Ph.D. in Chemistry and Physics of Condensed Matter from the University of Bordeaux I (France) in 2001. He came in the U.S. in 2002 for a post-doctoral visit at the University of Tennessee (Knoxville, TN) in the research group of Prof. Georges Guiochon. He worked there as a Research Scientist in the Department of Chemistry until 2014. In 2015, he joined Waters Corporation as a principal research scientist in the Instrument/Core research/Fundamental department. Dr. Gritti’s research interests involve liquid/solid adsorption thermodynamics and mass transfer in heterogeneous media for characterization and design optimization of new liquid chromatography columns/instrument technologies. He developed experimental protocols that helped (1) refine the models of adsorption isotherms used in preparative chromatography for the prediction of the band profiles of neutral and ionizable compounds, (2) decipher the mechanism of band broadening along modern analytical columns in linear chromatography, and (3) reshape modern columns and instruments to maximize performance in the field of separation science by liquid and supercritical fluid chromatography. Dr. Gritti has been invited to give 30 seminars, workshops, or tutorials on general chromatographic sciences at universities, discussion groups, or at international meetings. He has delivered about 50 invited keynote lectures at international Symposia and published over 270 peer-reviewed scientific papers. Dr. Gritti was the recipient of the 2013 Chromatographic Society Jubilee Medal for his contribution to the development of chromatographic science.

Gritti

ROSANA GUIJT, Professor, Smart Sensing, Centre for Rural and Regional Futures (CeRRF), Deakin University, Geelong, VIC, AUSTRALIA

3D Printing for Functionally Integrated Fluidic Devices

Bio: Rosanne M. Guijt studied BioPharmaceutical Sciences at Leiden University (The Netherlands), and was awarded her PhD degree in 2003 from Delft University of Technology (The Netherlands). As postdoctoral researcher, she initiated Lab-on-a-Chip research at the University of Tasmania (Australia) in the Australian Centre for Research on Separation Science (ACROSS). As an Alexander von Humboldt Fellow, she visited the prof Manz at the Korean Institute for Science and Technology Europe in Saarbrücken (Germany) in 2014/15 and the Microfluidic Chip Ship Jena (Germany) in 2016/17. Her specialty is the fabrication and use of functionally integrated microfluidic devices, including the integration of electrodes and porous materials. Since 2017, she has been a professor at Deakin University (Australia). Her research aims at the use of fluidics in the development of portable and field deployable analytical instrumentation, focusing on the development of new platforms for early detection of threats, spoilage, and disease.

GUIJT Rosanne at Deakin Univ (1)

DAVE HAGE, University of Nebraska-Lincoln, Lincoln, NE, USA

Ultrafast Affinity Extraction: New Developments in Strategies for Biointeraction Studies and the Analysis of Drugs or Hormones for Personalized Medicine

Bio: David S. Hage is the James Hewett University Professor in the Department of Chemistry at the University of Nebraska-Lincoln (Lincoln, Nebraska, USA). His research group is interested in theory, development, and use of affinity-based separations in HPLC, capillary electrophoresis, and mass spectrometry. Examples of his work include chromatographic-based immunoassays, microscale affinity separations, multi-dimensional affinity methods, and the use of immobilized biological agents in chiral separations or the study of biological interactions. Prof. Hage has over 310 papers, book chapters, and books in this field, along with seven U.S. patents. He has several awards for his work in these areas, including the 2021 American Chemical Society Award in Chromatography and the 2017 Pierce Award in Affinity Technology. He is current Editor-in-Chief for the Journal of Chromatography B, and the editor of the Handbook of Affinity Chromatography.

Studio portrait of David Hage, Professor, Chemistry. August 9, 2018. Photo by Greg Nathan, University Communication Photographer.

IMAD HAIDAR AHMAD, Associate Principal Scientist, Analytical Chemistry Enabling Technology, Process R&D, Merck Research Laboratories, USA

In silico Multifactorial Modeling for Streamlined Development and Optimization of Two-dimensional Liquid Chromatography

Bio: Dr. Haidar Ahmad received his Ph.D. from Florida State University under the mentorship of Dr. André Striegel focusing on macromolecular characterization. He completed his postdoctoral research with Prof. Peter Carr at University of Minnesota where he studied fundamentals of chromatography. He is currently an associate principal scientist and scientific supervisor in the Analytical Chemistry Enabling Technology group within Merck’s Process Research & Development department. Before his current role at Merck, he was a Principal Scientist at Novartis where he focused on building analytical capabilities for method development and characterization of inhalation drug products. Dr. Haidar Ahmad is experienced in macromolecular characterization, multidimensional liquid chromatography, method development, and use of software-assisted method development for modeling 1D and 2D-LC separations. Dr. Haidar Ahmad is the author of 40 publications, including four AChem papers in the last two years, with the work highlighted thrice in the journal front cover.

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MICHAL HOLČAPEK, Professor, Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, CZECH REPUBLIC

UHPSFC/MS as the Method of Choice for High-throughput Lipidomic Quantitation of Biological Samples

Bio: Michal Holčapek is a Professor at the University of Pardubice, Department of Analytical Chemistry. He received his Ph.D. in analytical chemistry from the same university in 1999. His research focus is mass spectrometry and its coupling with liquid chromatography or supercritical fluid chromatography, applied mainly in lipidomic analysis and cancer biomarker research. He obtained several scientific awards, for example, the Herbert J. Dutton Award (2022, American Oil Chemists' Society) and the Power List of 100 most influential people in the analytical sciences (2020, 2015 and 2013, The Analytical Scientist). He is a coauthor of 145 articles in peer-reviewed international journals, h-index 45, a contributing editor of Trends in Analytical Chemistry, a member of editorial advisory boards of Analytical and Bioanalytical Chemistry, and Lipids. He was coeditor of the books Handbook of Advanced Chromatography/Mass Spectrometry Techniques (Academic Press and AOCS Press, 2017) and Extreme Chromatography: Faster, Hotter, Smaller (American Oil Chemical Society, 2011), editor of topical collections in Analytical Chemistry, Analytical and Bioanalytical Chemistry, and Journal of Chromatography A, former head of Czech Mass Spectrometry Section and national representative in International Mass Spectrometry Foundation (2005-13), the chairman of over ten international and national conferences, e.g., HPLC 2017 Prague, 3rd European Lipidomic Meeting in 2013, etc. He is one of the founding members of the Lipidomics Standards Initiative and a vice-president for conferences in the International Lipidomics Society.

HOLCAPEK Michal

GERARD HOPFGARTNER, Professor, University of Geneva, Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, SWITZERLAND

Improving Sensitivity, Selectivity, and Information Content of Mass Spectrometry for the Analysis of Metabolites in Biological Samples 

Bio: Gérard Hopfgartner is a Professor for analytical chemistry and mass spectrometry at University of Geneva. He received his Ph.D. degree in 1991 in the field of organic geochemistry and mass spectrometry. Then he moved to Cornell University as a postdoctoral fellow working on LC-MS/MS. In 1992 he joined F. Hoffmann-La Roche in Basel until 2002 where he headed the bioanalytical section as an scientific expert. Up to 2015 he was Professor at the School of Pharmaceutical Sciences at UniGe and UNIL. His research interests focus on the development of novel mass spectrometry approaches with and without chromatography including analytical proteomics, metabolomics and toxicology. He is also one of the editor of Analytical Bioanalytical Chemistry.

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CHRISTIAN G. HUBER, Professor, University of Salzburg, Salzburg, AUSTRIA

When HPLC and MS Must Act in Concert: Determination of Fc-Glycosylation in Immunoglobulin G (IgG) Subclasses

Bio: CHRISTIAN HUBER trained as an analytical chemist at the University of Innsbruck focusing on chromatographic separation methods for biopolymers. After a postdoc in Csaba Horváth’s group at Yale University in 1996 focusing on building an instrument for capillary electrochromatography, he obtained lecturing qualification in analytical chemistry at the University of Innsbruck in 1997. As an associated professor of analytical chemistry at the University of Innsbruck from 1997 to 2002, he developed monolithic stationary phases for hyphenating high efficiency nucleic acid-, peptide-, and protein separations to mass spectrometry. From 2002-2008 he held a position as professor for analytical chemistry at Saarland University, where he started working in the field of proteome analysis and data mining of mass spectrometry data. Since 2008, he is a professor of chemistry for biosciences at the University of Salzburg. From 2013 to 2021 he led the Christian Doppler Laboratory for Biosimilar Characterization, which cooperated with Novartis and Thermo Fisher Scientific. His current research interests include proteome and metabolome analysis of biological models for disease as well as in-depth (therapeutic) protein characterization by means of HPLC and MS.

Huberapproved

MENG JING, Dow Inc., Collegeville, PA, USA

Applications of Asymmetrical Flow Field-Flow Fractionation and Capillary Electrophoresis in Characterization of Industrial Colloidal Particles

JING Meng at Dow

RYAN KELLY, Associate Professor, Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT, USA

Miniaturized LC Separations for Single-Cell Proteomics
Bio: Dr. Kelly earned his Ph.D. in analytical chemistry in 2005 from Brigham Young University (BYU) developing microfluidic separations for ultrasensitive bioanalysis. He spent the next 13 years at Pacific Northwest National Laboratory (PNNL), where he began as a postdoc and ultimately served as Chief Technologist of EMSL, PNNL’s national scientific user facility. He returned to BYU in 2018 as an associate professor, where he focuses on expanding the frontiers of single-cell and spatial proteomics. By combining expertise in microfluidics, separations and ultrasensitive mass spectrometry, Dr. Kelly’s team developed the nanoPOTS (Nanodroplet Processing in One pot for Trace Samples) platform, which is the first in-depth and label-free platform for single-cell proteomics. Kelly’s research group continues to advance this emerging field, with innovations across the workflow leading to continuous improvements in sensitivity, throughput and quantitative performance. Dr. Kelly has authored or coauthored more than 100 publications and is a named inventor on 14 issued and pending patents; several of these patents have been licensed and commercialized. His work has been recognized with numerous awards including two R&D 100 awards and the Georges Guiochon HPLC Faculty Fellowship.
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ROBERT KENNEDY, University of Michigan, USA

Capillary LC at 35,000 PSI and Beyond for Complex Mixture Analysis

Bio: Robert Kennedy is the Hobart H. Willard Distinguished University Professor of Chemistry and Professor of Pharmacology at the University of Michigan. He earned a PhD at University of North Carolina in 1988 where his work focused on using open tubular LC to analyze single cells. After a post-doc in neuroscience he started his own research program at University of Florida in 1991 before moving to University of Michigan as the Hobart H. Willard Professor of Chemistry in 2002. His research has combined his interest in biology with chemical analysis and separations. A theme of his group has been development of new chemical analysis tools that can be used at the nanoscale for several applications including screening of drugs, engineering enzymes, monitoring neurotransmitters in the brain, and studying the secretion of insulin and other hormones. His work has been recognized by several awards including the American Chemical Society Award in Chromatography, the Ralph Adams Award in Bioanalytical Chemistry, and NIH MERIT awards. He has held several service posts and is presently Associate Editor of Analytical Chemistry and Chair of the Chemistry Department at University of Michigan.

9/24/19 Chemistry Faculty portraits - Day2

CALEB KING, Analytical Chemist, Director of Process Development and Implementation, Front Range Biosciences, Boulder, CO, USA

Quantitative Analysis of Bioflavonoids in Cannabis spp

Bio: Caleb King directs analytical testing and phytochemical method development at Front Range Biosciences. Currently, Caleb works closely with a multi-disciplinary team working to understand the biogeochemistry and metabolomics of high-value crops. His focus on secondary metabolite identification has led to success in accurately quantifying cannabinoids, flavonoids, terpenoids, and alkaloids in Cannabis spp. using novel and efficient methodologies. He is also experienced in mass spectrometry and compound identification that has led to peer-reviewed publications, book chapters, and presentations. Caleb received his Master of Science in marine sciences from the University of North Carolina at Chapel Hill and his Bachelor of Science from the University of Mount Union. He has over 10 years of combined experience in paleoclimate research, environmental chemistry, veterinary medicine, and cannabis science.

King, Caleb

REIKO KIYONAMI,Senior Applications Product Specialist, Pharma & Biopharma Vertical Marketing, Thermo Fisher Scientific, San Jose, CA, USA

Characterization and Quantification of Lipid Nanoparticle Components and Their Degradants in vivo using an LC-HRAM MS Platform

Bio: Reiko Kiyonami is the Senior product applications specialist at Thermo Fisher Scientific. She joined Thermo Fisher Scientific in 1990 and has extensive hands-on experiences for characterization and quantification of large biologics and small molecules. Her recent focus is to develop advanced analytical solutions for new therapeutic modalities using Orbitrap based mass spectrometry.

Kiyonami

EBERHARDT KUHN, Marketing Manager, Shimadzu Scientific Instruments, Columbia, MD, USA

Delta Dawn - the Next Generation of Cannabinoids?

Bio: Dr. Eberhardt Kuhn is the Marketing Manager for Food, Cannabis, and Hemp at Shimadzu Scientific Instruments.   He is a renowned chromatographer with over 30 years of experience in this field at companies like Restek, Agilent, and Bio-Rad.  Dr. Kuhn has presented talks at conferences and events in over 30 countries.  His expertise is the field of chromatographic separations, column chemistry, and scientific instrumental analysis of food, including determination and identification of contaminants (pesticides, heavy metals, and Food Contact Materials, such as PFAS), food quality, food adulteration and confirmation of food authenticity, and general analytical method development.   His recent interests focus on all aspects of cannabis and hemp analytics, from potency to terpenes to contamination (pesticides, residual solvents, metals), as well as educating the public on the countless benefits of hemp and cannabis. 

Kuhn, E

STEFAN LAMOTTE, Senior Principal Scientist Liquid Chromatography, Analytical & Material Science, LC, Bioanalysis & Chemometrics, BASF SE, Ludwigshafen, GERMANY

The Good, the Bad and the Ugly - Towards the Separation of Polar Analytes in Supercritical Fluid Chromatography

Bio: Studying chemistry at Saarland university Saarbruecken, Diploma Thesis in 1995 in the field of HPTLC; PhD working group and field of work: Prof. Heinz Engelhardt, Saarland University (1998) in the field of synthesis and application of sub 2 μm nonporous particles for use in fast HPLC; 1998-2010 Division manager columns & stationary phases Bischoff Chromatography; Since 2011: Competence Center analytics, BASF SE, Ludwigshafen, Germany; Since 2012 Member of the expert group of the general chromatography chapter (CST-Expert working party) of the European Pharmacopoeia Authority (EDQM); Since 2019 Deputy Chairman of the Working Party Separation Science of the Department of Analytical Chemistry of the German Chemical Society (GDCh); Gerhardt Hesse Award of the Working Party Separation Science of the Department of Analytical Chemistry of the German Chemical Society (GDCh) in 2019

LAMOTTE Stefan at BASF

MILTON L. LEE, Emeritus H. Tracy Hall Professor of Chemistry, Brigham Young University, and Chief Science Officer, Axcend, Provo, UT, USA

Implementation of Compact Capillary LC into Analytical Workflows

Bio: Milton L. Lee received a B.A. Degree in Chemistry from the University of Utah and a Ph.D. in Analytical Chemistry from Indiana University. He was a postdoctoral researcher at MIT before accepting a faculty position at Brigham Young University. Dr. Lee’s research centers on capillary separation techniques and mass spectrometry detection. He has co-authored over 600 publications and has received various awards including the ACS Award in Chromatography, ACS Award in Chemical Instrumentation, Pittsburgh Analytical Chemistry Award, ACS Award in Separations Science and Technology both LCGC Europe and North America Lifetime Achievement Awards, and the 2019 ACS Award in Analytical Chemistry. Dr. Lee has mentored over 100 graduate students and post-doctoral researchers. Professor Lee is also an entrepreneur and has been involved in transferring technology from his university research laboratory to the private sector, co‑founding four analytical instrument companies. He was named five times on the Analytical Scientist Power List, the latest in 2021.

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FREDERIC LYNEN, Separation Science Group, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Ghent, BELGIUM

Overcoming Separation and Detection Challenges in 1-D LC and in LCxLC through Purely Aqueous Temperature Responsive Liquid Chromatography

Bio: Frédéric Lynen is full professor and head of the Separation Science Group in the Department of Organic and Macromolecular Chemistry at Ghent University. His research comprises chromatographic column technology, temperature responsive liquid chromatography, stationary phase synthesis, green chromatography, multidimensional separations, chromatographic predictive modelling and high-end implementations of hyphenated techniques in chromatography in the fields of environmental, pharmaceutical or art-historical analysis. He is also active in the development of LC-MS and GC-MS based non-targeted approaches allowing e.g. biomarker discovery. He authored over 140 journal articles in the field of separation sciences and was awarded the Jubilee Medal by the Chromatographic Society in 2019 for his work.

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TODD MALONEY, Executive Director, Synthetic Molecule Design and Development, Eli Lilly and Company, Indianapolis, IN USA

Chromatography at the Interfaces: Lessons Learned and Future Trends in Online HPLC for Reaction Monitoring and Advanced Process Understanding and Control

Bio: Todd D. Maloney earned his B.S. in Chemistry in 1996 from the State University of New York at Oswego, and his Ph.D. in Chemistry in 2001 from the University at Buffalo, The State University of New York. He started his career in 2001 with Pfizer Global Research and Development in Ann Arbor, MI. While at Pfizer he was a member of the Chemical R&D and Analytical Sciences R&D organizations, where he was involved in early phase analytical development, automation technologies, and in-silico method development strategies for pharmaceutical analysis. In 2007 he joined Eli Lilly and Company and is currently an Executive Director in the Synthetic Molecule Design and Development group where he leads teams developing process analytical technologies and integrated process control strategies for oligonucleotides, peptides, and small molecules.

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KATARINA MARAKOVA, Assistant Professor, Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, SLOVAKIA

Intact Proteins Analysis from Biological Samples by Liquid Chromatography – Triple Quadrupole Mass Spectrometry with Solid Phase Extraction as Sample Pretreatment Method

Bio: Katarína Maráková is an Assistant Professor at the Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava. Her research interests focus on high-performance separation methods, including multidimensional approaches and their coupling to mass spectrometry, for advanced pharmaceutical, biomedical applications, and chiral analysis. Her main research activities involved the development of capillary electrophoretic methods for the analysis of small molecules (drugs, metabolites). During her recent post-doctoral research stays at the University of Texas at Arlington (prof. K.A. Schug), supported by the Fulbright Commission and the National Scholarship program of the Slovak Republic, she developed liquid chromatography-mass spectrometry methods for the analysis of intact proteins. Her current research is also directed toward new sample preparation methods for the intact protein analysis from complex biological matrices.

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DAVID MCCALLEY, Professor in Bioanalytical Science, Department of Health and Applied Sciences, University of the West of England, Bristol, U.K.

Managing Deleterious Solute Interactions with Metals in Hydrophilic Interaction Chromatography

Bio: David McCalley is Professor of Bioanalytical Science at the University of the West of England, U.K. In 2021, he was named as one of the world's 100 most influential analytical scientists by 'Analytical Science' magazine. He was awarded the Silver Jubilee medal of the Chromatographic Society in 2008. He serves on the Editorial Board of the Journal of Chromatography A and the magazine LC.GC. In the past five years, he has given invited lectures in Ghent, Cannes, Princeton, London, San Francisco, Paris, Prague, Lake Balaton and Sandefjord. Professor McCalley's research is directed towards the understanding of the fundamental mechanisms of separation that occur in liquid chromatography. These studies in LC have included the effects of pressure and temperature on retention and efficiency, mixed mechanisms for strongly basic compounds, performance of superficially porous packings, and overloading effects in both reversed-phase and hydrophilic interaction chromatography. Applications have included monoclonal antibodies, peptides, natural products, steroids, pharmaceuticals and clinically relevant compounds using both UV detection and mass spectrometry.

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R. KEN MARCUS, Robert Adger Bowen Professor of Chemistry, Department of Chemistry, Clemson University, Clemson, SC, USA

High Throughput and High Purity Exosome Isolations using Capillary-Channeled Polymer (C-CP) Fiber Phases

Bio: Dr. Marcus earned B.S. degrees (1982) in chemistry and physics from Longwood College and a Ph.D. (1986) in analytical chemistry from the University of Virginia. He serves on the editorial advisory board for three international journals and was the recipient of the 2001 S.C. Governor¹s Award for Excellence in Science Research. In 2010, Professor Marcus was named a Fellow of the Royal Society of Chemistry (FRSC), in 2012 a Fellow of the American Association for the Advancement of Science (FAAAS), in 2016 a Fellow of the Society for Applied Spectroscopy, and in 2018 a Fellow of the National Academy of Inventors. In 2019, Marcus was awarded the inaugural Clemson University Researcher of the Year designation. Marcus has been on the Clemson faculty for 35 years, graduating 41 Ph.D. and 12 MS students.

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YEHIA MECHREF, Paul W. Horn Distinguished Professor, Associate Vice President of Research and Innovation, and Director of the TTU Center for Biotechnology & Genomics, Texas Tech University, Lubbock, TX, USA

LC-MS/MS of Glycan and Glycopeptide Isomers using Mesoporous Graphitized Carbon Column

Bio: Prof. Yehia Mechref is a Paul W. Horn Distinguished Professor in the Department of Chemistry and Biochemistry at Texas Tech University, an Associate Vice President for Research and Innovation, and the Director of the Center for Biotechnology and Genomics. He received a B.Sc. in chemistry from the American University of Beirut and a Ph.D. with an honorable mention from Oklahoma State University (Stillwater, OK, USA). Dr. Mechref is a world-renowned scientific leader in developing sensitive biomolecular mass spectrometry methods enabling qualitative and quantitative assessments of the roles of proteins, glycoproteins, and glycans in the development and progression of numerous diseases and medical conditions. Dr. Mechref is a prolific writer who has published 35 review articles, 16 book chapters, and 216 peer-reviewed research papers. Currently, Dr. Mechref’s Google Scholar h-index is 67, with 13,404 citations. Dr. Mechref is the founding Editor-in-Chief of BioChem, Associate Editor of Frontiers Oncology, and a standing member of the NIH EBIT study section. Dr. Mechref currently co-chairs the Steering Committee of the NCI Alliance of Glycobiologists for Cancer Research. Dr. Mechref is the recipient of numerous awards, including the 2019 TTU President’s Academic Achievement Award, and the 2019 TTU Nancy Bell Faculty Excellence in Mentoring Award.

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HE MENG, Associate Director, Analytical Development, Global CMC Development, Sanofi, Framingham, MA, USA

An Analytical Quality by Design Approach for Global Platform Analytical Method Development and Qualification to Support Accelerated CMC Development

Bio: He Meng is an Associate Director in Analytical Development in Sanofi Global CMC Development. He is responsible for the development, qualification and validation of physicochemical analytical methods using an AQbD approach to support the release/stability testing and characterization of biologics in preclinical and clinical development, including antibodies, enzyme replacement therapies, protein conjugates, and gene therapies. He serves as the analytical lead for a Phase I mAb program for the treatment of solid tumors. He also contributes to the preparation of CTD analytical sections for an enzyme replacement therapy for global regulatory filing for marketing approval.

He Ming

GREGORY NORDIN,Professor, Brigham Young University, Provo, UT, USA

High Resolution 3D Printing for Microfluidics

Bio: Gregory P. Nordin did his PhD in Electrical Engineering at the University of Southern California (1992) after earning BS and MS degrees in Physics at Brigham Young University (1984) and UCLA (1986), respectively. From 1984 to 1992 he also worked at the Hughes Aircraft Company. From 1992 to 2005 he was in the Electrical and Computer Engineering Department at the University of Alabama in Huntsville where he was founding director of the university's Nano and Micro Devices Center. In 2005 he joined BYU as a professor in the Electrical & Computer Engineering Department where his research focuses on 3D printing for microfluidics, and micro- and nano-fabricated devices for biosensing, photonics, and MEMS.

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LUCIE NOVAKOVA, Professor, Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Hradec Králové, CZECH REPUBLIC

Problems of Stationary Phases in Supercritical Fluid Chromatography

Bio: Lucie Nováková is a full Professor in Analytical Chemistry at the Charles University, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, the Czech Republic since 2011. Her research is oriented towards separation techniques, namely ultra-high performance liquid chromatography, supercritical fluid chromatography, and their coupling to mass spectrometry. She is involved in a broad scope of research projects focused on pharmaceutical analysis, doping control, plant analysis, and bioanalytical methods. An important part of her research also lies in the sample preparation step, where the focus is put on the current trends enabling facilitation, miniaturization, and reduction of time and sample requirements. She extended her scientific experience during the fellowships at world-recognized universities, such as the University of Geneva and Vrije Universiteit Brussel, beyond others. She authored two books on HPLC theory and practice and nine book chapters. She published over 125 peer-reviewed scientific articles and review papers with more than 4160 citations and an h-index of 35. She is also widely involved in teaching and education activities, such as HPLC and SFC training courses, seminars, and conferences. Currently, she is the principal team manager of the STARSS project. 

doc. PharmDr. Lucie Nováková, Ph.D. - Fotografováno v Hradec Králové 8.7.2016.

KOJI OTSUKA, Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, JAPAN

Strategy for using Specific Interactions in Microscale HPLC

Bio: Koji Otsuka is a professor of Analytical Chemistry of Materials, Department of Material Chemistry, Graduate School of Engineering, Kyoto University.  He received his Ph.D. from Kyoto University in 1986.  After the JSPS Fellowship for Young Scientists (1986–1988), he joined Osaka Prefectural College of Technology as a senior lecturer (1988–1990) and an associate professor (1990–1995), then moved to Himeji Institute of Technology as an associate professor of the Prof. Terabe’s Lab (1995–2002).  In 2002 he joined Kyoto University as a full professor. His research interests include the development of micro/nano scale high performance separation techniques using electrophoretic and chromatographic methods, and their application studies mainly in bio-related and environmental analyses.  He has published approximately 200 papers and book chapters, cited over 10000 times. He received the Award of the Society for Chromatographic Sciences in 2006, the Award for Creative Work of the Chemical Society of Japan in 2009, and the Award of the Japan Society for Analytical Chemistry and the Terabe Shigeru Prize in 2014.  He is a member of the Permanent Scientific Committees of the HPLC and ITP Symposia and serves as editors of the Journal of Separation Science (JSS) and Separation Science Plus (SSCP) (Wiley-VCH).

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BRETT PAULL, Professor, Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences (Chemistry), University of Tasmania, Hobart, AUSTRALIA

Taking the Chromatograph Out of the Lab: High and Low Pressure Field Deployable Instruments for Environmental Applications

Bio: Brett Paull is a University of Plymouth (UK) B.Sc. (Hons), Ph.D. and D.Sc. graduate, and a Professor of Analytical Chemistry in the School of Natural Sciences, within the College of Sciences and Engineering at the University of Tasmania. Brett took up his first lectureship at the University of Tasmania from 1995 to 1997, before moving to Dublin City University (DCU), Ireland, as Lecturer (1998-2003), Senior Lecturer (2003-2006) and Associate Professor (2006-2011). In 2011 Brett rejoined the University of Tasmania as a Professor of Analytical Chemistry under the University's New Stars program. From 2014 to 2019 Brett was Director of the Australian Centre for Research on Separation Science (ACROSS), and from 2015-2020 the Director of the ARC Training Centre for Portable Analytical Separation technologies (ASTech). Professor Paull’s research interests lie in the development and characterization of advanced materials for application within the analytical sciences, and the development of advanced methods and technologies for industrial and environmental analysis. His research is detailed within over 330 published research articles, papers and book chapters/books, which collectively have been cited over 9,500 times to-date (GS).

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JOSEPH J. PESEK, Professor, Chemistry, San Jose State University, San Jose, CA, USA

How to Facilitate Method Development for Hydrophilic Compound

Bio: Joseph Pesek, emeritus professor of analytical chemistry at San Jose State University, (B.S. chemistry, the University of Illinois, 1966 and Ph.D. in analytical chemistry, UCLA, 1970) and a postdoctoral fellow at UCLA.  Academic appointments were at Northern Illinois University, 1971 and San Jose State University, 1978.  He was chair of the Chemistry Department and Associate Dean for Graduate Studies and Research, visiting research professor at Ecole Polytechnique, in Paris, Universite d’Aix-Marseilles, Monash University in Melbourne, Australia and the University of Queensland Department of Pharmacy. Honors include Camille and Henry Dreyfus Scholar in 1991 and 2001, President’s Scholar at San Jose State and EAS lifetime award in chromatography.  He received 60 research grants totaling over $6.7 M.  Research interests include the synthesis and characterization of separation materials for chromatography and applications in biological, medicinal, clinical, forensic, pharmaceutical and food analysis. He has 250 publications in peer-reviewed scientific journals and 3 patents. 

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SEBASTIAN PIENDL, University of Leipzig, GERMANY

Multidimensional Separations in Microfluidic Glass Chips

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ROBERT PLUMB, Director Metabolic Phenotyping, Health Sciences, Waters Corporation, Milford, MA, USA

Chromatographic Advances in LC/MS based Metabolic Profiling

Bio: Dr Robert Plumb is the Director of Omics and Small Molecule Pharma in the Waters Scientific Operations Division, Milford, Massachusetts, responsible for the development of new approaches to DMPK, proteomics and metabolomics. Prior to his current position he GlaxoSmithKline for 15 years in the areas of bioanalysis, drug metabolism and metabolic profiling. Dr Plumb has published over 100 papers on the subject of HPLC/MS and NMR for bioanalysis, metabolomics and metabolite identification.  He is a Adjunct Professor at Murdoch University and a Fellow of the Royal Society of Chemistry.  In 2014 he was awarded Highly Cited Researcher by Thompson Reuters.

PLUMB Robert at Waters Corp

CHRISTOPHER POHL, Chromatography Consultant, CAP Chromatography Consulting, Union City, CA, USA

Recent Developments in the Synthesis of High Performance Anion Exchange Materials based on Hyperbranched Polymers

Bio: Christopher Pohl is a chromatography consultant for CAP Chromatography Consulting. He retired from Thermo Fisher Scientific in August 2021 where he was Vice President, Chromatography Chemistry in the Chromatography & Mass Spectrometry Division of Thermo Fisher Scientific.  He was actively involved in chromatography consumables R&D.  Christopher joined Dionex now part of Thermo Fisher Scientific in 1979 where the focus of his work has been new stationary phase design.  He is an author or co-author of 102 US patents, along with numerous foreign equivalents, in a number of areas including separation methods, stationary phase design, suppressor technology, solid-phase extraction, capillary electrophoresis techniques, and accelerated solvent extraction (ASE).  He is the author or co-author of 14 book chapters and 149 papers published in scientific journals.  He received his BS in Analytical Chemistry from the University of Washington in 1973. He received the International Ion Chromatography Symposium Award in 1990 in recognition of his contributions in developing the methodology of ion chromatography. He received the Uwe Neue Award and the Eastern Analytical Symposium Separation Science Award in 2018 in recognition of his contributions to separation science. Most recently he received the 2020 Thermo Fisher Scientific - George N. Hatsopoulos technical innovation award.

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ELLA PSILLAKIS, Professor, School of Chemical and Environmental Engineering, Chania-Crete, GREECE

The Ten Principles of Green Sample Preparation and the First Metric Tool for Greenness Assessment

Bio: Elia Psillakis (EP) is Full Professor in Water Chemistry at the School of Chemical and Environmental Engineering, Technical University of Crete, Greece. Her research activities focus on (i) the fundamentals and the development of new sample preparation methods, (ii) the fate of organics in natural and engineered water and (iii) green sample preparation. She has received a Fulbright Award and used it at Caltech, USA. She was featured in the Power List 2021 of the “The Analytical Scientist”. She has 6 book chapters, 100 ISI publications with > 7500 citations (h-index=45) and 3 “Top cited article” awards. EP is Editor-in-Chief of “Advances in Sample Preparation” (Elsevier), Specialty Chief Editor of “Environmental Analysis” (Frontiers) and Editor in “Journal of Separation Science” (Wiley). EP is Head of the Sample Preparation Study Group and Network of the EuChemS-DAC. She is Director of a Spin-Off Company. From 2014-2016 she acted as the Deputy Rector of Academic Affairs and Research.

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ERIK REGALADO, Merck, USA

Trapping-enrichment Multi-dimensional Liquid Chromatography with On-line Deuterated Solvent Exchange for Streamlined Structure Elucidation at the Microgram Scale

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CLAUS RENTAL, Executive Director, Ionis Pharmaceuticals, Carlsbad, CA, USA

Analytical Techniques for Oligonucleotide Therapeutics

Bio: Claus Rentel has more than 20 years of experience in quality control, analytical development and method validations. He has extensive expertise in the development of oligonucleotide therapeutics in regard to specifications, testing of starting materials and reagents, drug substance intermediates, drug substance, drug product and toxicological samples, as well as validation of IT quality systems. He has been responsible for the CMC drug substance section of IND filings for more than 40 oligonucleotide therapeutics, and participated in the NDA filings for KYNAMRO® (mipomersen), SPINRAZATM (nusinersen), WAYLIVRA® (volanesorsen), and TEGSEDITM (inotersen). Claus is currently Executive Director, Analytical Development and Quality Control at Ionis Pharmaceuticals, Inc., Carlsbad, California. Prior to joining Ionis in 2001 he worked in Quality Control and Special Analytics at CarboGen Laboratories AG, Aarau, Switzerland. He received his Ph.D. (summa cum laude) from the University of Tübingen, Germany.

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JEAN-MARC ROUSSEL, JM Roussel Consulting, Macon, FRANCE

Improving the Interpretation of Robustness Studies: Managing the Leap into the Real World

Bio: Jean-Marc Roussel is, since 2002, an independent consultant who helps industry laboratories in developing and validating their analytical methods. His consultancy activity also includes lectures and trainings related to liquid chromatography (analytical and preparative), sample preparation and statistics applied to analytical chemistry (validation, robustness, uncertainty, method transfer…). He is, together with Prof. M. Righezza, designer of NeoLiCy®, software for analytical method’s life cycle statistical assessment. Before starting a consultancy activity, he has been for 14 years Technical Support and Development Manager for laboratory suppliers in France. He serves as a volunteer in the United States Pharmacopeia (USP), “Measurement and Data Quality” Committee of Experts, and French Society for Pharmaceutical Sciences and Techniques (SFSTP), as chairman of the “Uncertainty of Measurement” and Co-Chairman of the “Analytical Methods Robustness” committees. He holds a Ph.D. (1988, Prof. A.M. Siouffi) from Aix-Marseille university and has been associate to Paris-Saclay university for 12 years.

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KOEN SANDRA, CEO and Co-owner at RIC group, Kortrijk, BELGIUM; Visiting Professor, Ghent University Ghent, BELGIUM

Recent Trends in the Analysis of Biopharmaceuticals

Bio: Koen Sandra received a PhD degree in Biochemistry from the Ghent University, Belgium in 2005. After his PhD, he joined Pronota, a molecular diagnostics company where he was active in developing analytical platforms for disease biomarker discovery and in setting up external collaborations. In 2008, he joined RIC, a company that provides chromatographic, electrophoretic and mass spectrometric support to the chemical, life sciences and pharmaceutical industries, where he holds the position of CEO. As a non-academic scientist, Koen Sandra is author of over 50 highly cited scientific papers and has presented his work at numerous conferences as an invited speaker.

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KEVIN A. SCHUG, Department of Chemistry & Biochemistry, University of Texas Arlington, USA

Liquid Chromatography – Tandem Mass Spectrometry for Discovery and Profiling of Hemp (Cannabis) Anthocyanins

Kevin A. Schug is Professor and the Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington (UTA).  He is also a Partner with Medusa Analytical, LLC, a consulting company.  He received his B.S. degree in Chemistry in 1998 from the College of William and Mary, and his Ph.D. degree in Chemistry from Virginia Tech in 2002.  From 2003-2005, he performed post-doctoral research at the University of Vienna in Austria. Since joining UTA in 2005, his research has been focused on the theory and application of separation science and mass spectrometry for solving a variety of analytical and physical chemistry problems, in the fields of forensic, environmental, pharmaceutical, biological, and energy research.  He has over 200 peer-reviewed publications and over 600 presentations, posters, and invited talks to his group’s credit.  Dr. Schug has received several research awards, including the 2009 Emerging Leader Award in Chromatography by LCGC Magazine and the 2013 American Chemical Society Division of Analytical Chemistry Young Investigator in Separation Science Award. He was named to the 2019 and the 2021 The Analytical Scientist’s Top 100 Power List of the most influential analytical chemists in the world. 

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MARK SCHURE, Chief Technology Officer, Kroungold Analytical, Inc., Blue Bell, PA, USA

Size-exclusion in Capillary Chromatography: Simulation and Experiment

Mark recently went from part time consulting while spending 28 years in industry (Digital Equipment Corporation, Rohm and Haas Company and The Dow Chemical Company) to full-time consulting. Mark has held a leadership role in separation science for many years in areas which included the interface between separation science and mechanistic investigations using high performance computing. Specifically, Mark's interests include theoretical and experimental separation science with specific interests in mass transport phenomena, retention mechanisms at the molecular level and in macromolecular separation mechanisms. In addition, he has worked in computational chemistry, statistical physics, high performance computing hardware and software development including parallel processing, numerical methods, and database application development. He has also made contributions in polymer and colloid chemistry and laboratory automation including high-throughput systems and chemometrics.

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STEPHANIE A. SCHUSTER, Senior Technical Support Scientist, Advanced Materials Technology, Inc., Wilmington, DE, USA

New 1.5 mm UHPLC Columns Enable Robust Separations with Increased Sensitivity and Solvent Savings

Bio: Dr. Schuster earned her Ph.D. under the guidance of Prof. Joe P. Foley at Drexel University. She joined Advanced Materials Technology, Inc. (AMT) in June 2009 as a research scientist working with Dr. Jack Kirkland, providing contributions to the commercial development of products designed for peptide and protein separations.  Most recently, as part of the Technical Support team at AMT, Stephanie has been providing customers support in optimizing and trouble-shooting their applications.   

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MARCELA SEGUNDO, Professor, LAQV/REQUIMTE, Faculty of Pharmacy, University of Porto, Porto, PORTUGAL

Bead Injection Concept: How Automation can Change Sample Treatment for Chromatography

Bio: Marcela Segundo holds a BSc in Microbiology and a PhD in Biotechnology (College of Biotechnology, Portuguese Catholic University). She is currently the Head of the Analytical Development Group at LAQV/REQUIMTE, Portuguese Government Associate Laboratory for Green Chemistry, Clean technologies and also member of the Executive Board of Faculty of Pharmacy, University of Porto, where she teaches in the Department of Chemical Sciences. Her current research is focused on automation of sample treatment and on development of high-throughput microchemical methods. Her scientific production (ORCID 0000-0003-2938-0214) comprises 14 book chapters, >140 peer-reviewed papers in international journals (h-index 31). In 2016 she received the FIA Award for Science attributed by the Japanese Association for Flow Injection Analysis. Since 2018, she is the Secretary of the Division of Analytical Chemistry of the European Chemical Society (EuChemS).

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STEVEN SOPER, University of Kansas, Lawrence, KS, USA

Nanoscale Electrophoresis as an Approach for Single-molecule DNA and RNA Sequencing

Bio: Prof. Steven A. Soper received his Ph.D. from the University of Kansas in 1989 followed by a Postdoctoral Fellowship at Los Alamos National Laboratory, where he worked on single-molecule detection for high speed sequencing of the human genome. In 1991, he joined the faculty at Louisiana State University (LSU) within the Department of Chemistry, where he filled the William H. Pryor Distinguished Chair of Chemistry. Prof. Soper also held joint appointments in Mechanical Engineering and Biological Sciences. While at LSU, he founded the Center of BioModular Multi-Scale Systems for Precision Medicine, which has as its primary charge to develop enabling and transformative tools for making health-related measurements from rare disease markers, such as liquid biopsy markers, with full process automation. This Center has recently been awarded funding from the National Institutes of Health as part of their Biotechnology Resource Center Program (funded through the National Institute of Biomedical Imaging and Bioengineering). In 2011, Prof. Soper accepted a position within the Department of Biomedical Engineering and Department of Chemistry at the University of North Carolina, Chapel Hill. Prof. Soper is currently a Foundation Distinguished Professor in Chemistry and Mechanical Engineering at the University of Kansas, Lawrence. Prof. Soper also holds an appointment at Ulsan National Institute of Science and Technology in Ulsan, South Korea, where he is a World Class University Professor. He is also serving as a Science Advisor for a number of major worldwide companies. Prof. Soper is currently the Editor of the Americas for the Analyst and on the Editorial Board for Journal of Fluorescence and Journal of Micro- and Nanosystems. Prof. Soper is also serving as a permanent Member of the Nanotechnology study panel with the National Institutes of Health. As a result of his efforts, Prof. Soper has secured extramural funding totaling >$93M, has published over 245 peer-reviewed manuscripts (h index = 66; 15,289 citations); 31 book chapters and 71 peer-reviewed conference proceeding papers, and is the author of 12 patents. He is also the founder of a startup company, BioFluidica, which is marketing devices for the isolation and enumeration of circulating tumor cells. Soper recently founded a second company, Digital NanoGenetics (DNG), which is seeking to market a new DNA/RNA single-molecule sequencing platform. His list of awards includes Chemical Instrumentation by the American Chemical Society, the Benedetti-Pichler Award for Microchemistry, Fellow of the AAAS, Fellow of Applied Spectroscopy, Fellow of the Royal Society of Chemistry, R&D 100 Award, Distinguished Masters Award at LSU and Outstanding Scientist/Engineer in the state of Louisiana in 2001. Finally, Prof. Soper has granted 46 PhDs and 6 MS degrees to students under his mentorship. He currently heads a group of 15 researchers.

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DWIGHT STOLL, Professor, Gustavus Adolphus College, Saint Peter, MN, USA

A Strategy for Assessing Peak Purity of Pharmaceutical Peptides in ReversedPhase Chromatography Methods using 2D-LC-MS

Bio: Dwight Stoll is Professor of Chemistry at Gustavus Adolphus College in St. Peter, MN. He has authored or co-authored more than 80 peer-reviewed publications and five book chapters in separation science, and speaks internationally on the topic. He has also written the monthly “LC Troubleshooting” column for LCGC Magazine since 2017. His primary research focus is on the development of two-dimensional liquid chromatography (2D-LC) for both targeted and untargeted analyses. Within this area he has made contributions on many aspects of the technique including stationary phase characterization, biopharmaceutical analysis, new 2D-LC methodologies and instrumentation, and fundamental aspects including re-equilibration in gradient elution liquid chromatography and analyte focusing. He has taught several short courses on 2D-LC in recent years at venues including Pittcon and the international HPLC20XX series. He is the 2011 recipient of LCGC’s Emerging Leader in Chromatography Award, the 2015 recipient of the American Chemical Society Division of Analytical Chemistry Award for Young Investigators in Separation Science, and in 2017 he received the Georges Guiochon Faculty Fellowship.

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ULRICH TALLAREK, Full Professor, Department of Chemistry, Philipps-Universität Marburg, Marburg, GERMANY

Multiscale Simulation of Mass Transport in Chromatographic Media: From Interfacial Dynamics to Hierarchical Porosity

Bio: Ulrich Tallarek is Professor of Chemistry at the University of Marburg (Germany). He obtained his PhD in Chemistry from the University of Tübingen in 1998 (advisors: Ernst Bayer and Georges Guiochon). After postdoctoral research at Wageningen University & Research (The Netherlands), he completed his habilitation at the Otto von Guericke University Magdeburg in 2004 and received a venia legendi for physical chemistry (mentor: Andreas Seidel-Morgenstern). His research centers on discovering and understanding fundamental morphology–functionality–transport relationships in porous media of practical relevance. The techniques used by his group include advanced simulations of fluid flow and mass transport in porous media, molecular dynamics simulations of solute behavior at solid‒liquid interfaces, and the physical reconstruction of porous media morphologies relying on various tomographic methods.

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ANTHONY TORRES, Front Range Biosciences, Oakland, CA, USA

Testing a Genetic Model for Chemotype Inheritance in Cannabis Sativa from a Hybrid Propyl Cannabinoid Breeding Population

Bio: Mr. Torres carries out biochemical and genetic research at Front Range Biosciences. He utilizes many techniques for molecular analysis of Cannabis, including HPLC. Prior to joining Front Range Biosciences, Anthony began his career in the cannabis industry at Steep Hill labs in 2013 where he worked in several roles as a scientist supporting research and development, laboratory method licensing, and production operations. Anthony received his education from the University of San Francisco for his undergraduate and graduate studies where he studied Molecular Biology. He is passionate about spreading the knowledge and furthering the advancement of cannabis science and genetics.

 

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FAROOQ WAHAB, Research Engineering Scientist, Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, USA

Current State of Ultrafast Chiral/Achiral Separations

Bio: M. Farooq Wahab is a Research Engineering Scientist at the University of Texas at Arlington. He did his Ph.D. from the University of Alberta, Canada, and a postdoctoral fellowship with Daniel W. Armstrong at the University of Texas at Arlington. Currently, he is working on hyphenating microwave rotational spectroscopy with gas chromatography, focusing on signal enhancement approaches to provide a sensitive detection technology for volatile isobaric, isotopic, and isomeric compounds. He also works on time and frequency domain signal processing techniques for noise suppression and resolution enhancement algorithms. Wahab was listed among “Top 40 Under 40 Analytical Chemists” by the Analytical Scientist Magazine (UK). He received the “Young Investigator Award” from the Chinese American Chromatography Association in Pittcon in 2019. The Journal of Separation Science included him among “Emerging Thought Leaders in Separation Science” in 2020. He is a reviewer for several leading analytical chemistry publications and volunteers for the ACS Division of Analytical Chemistry Education Committee.

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SISSI WHITE, Thermo Fisher Scientific, Franklin, MA, USA

Characterization of Lipid Nanoparticle (LNP) Composition using UHPLC-CAD

Bio: I am a Sr. Application Scientist of application team within the Chromatography and Mass spectrometry division of Thermo Fisher Scientific.  I am responsible for developing and validating chromatographic methods and solutions to meet biopharmaceutical customers’ needs. I have 15+ years analytical and project management experience with HPLC (Micro-scale UPLC, UPLC, UPC2, HDX and HPLC, SFC and Prep LC) and LC-MS (small molecule, amino acids, peptides, LNP and oligos) within the pharmaceutical industry and within instrumentation vendors. I received my MSc degree in Food Science from University of Massachusetts, Amherst and B.Sc. in Applied Chemistry from Qingdao Institute of Chemical Technology, China.  After graduation, I joined Armstrong Pharmaceuticals, Inc as Sr. Chemist to perform analytical testing in support of the release and stability testing of inhalation drugs in a cGMP laboratory.  Prior to Thermo Fisher, I worked as Sr. Evaluation Scientist in the Separations Evaluation group and later in the Systems Design & Development group at Waters Corporation for 13 years, where I drove hands-on evaluation project management for the delivery of ACQUITY M-Class, ACQUITY ARC, SFC, SFE, RenataDX IVD Screening and ACQUITY Arc 2D LC systems across multiple chromatographic data systems. Some of my responsibilities included providing expertise and resources to support design and implementation of experiments to assess new technologies, developing analytical methods to validate and ensure system performance, and driving validation and verification work to successful completion to support new products.

Sissi

STEVEN RAY WILSON, Professor, Department of Chemistry, University of Oslo, Oslo, NORWAY

Organoids, Organ-on-a-chip, and Liquid Chromatography

Bio: Steven Ray Wilson is a professor in analytical chemistry, specializing in separation science and mass spectrometry. Key themes in his research are on-line sample preparation and analysis of limited biosamples. His current focus is applying these approaches to studying organoids, i.e. lab-grown organs (drug metabolism, proteomics). Wilson is a PI in a Center of Excellence on this topic (Hybrid Technology Hub). He has several other active collaborations within medical research, e.g. clinical studies of inborn metabolic diseases, and brain cancer. Wilson has previously developed and applied a number of hyphenated systems/online sample preparation, such as 2D-LC, LC-NMR and IMER-LC-MS. Wilson has also worked with a number of columns formats such as open tubular LC and monoliths.
Wilson

JOHN R. YATES, III, Ernest W. Hahn Professor, Departments of Molecular Medicine and Neurobiology, The Scripps Research Institute, LaJolla, CA, USA

Single Cell Proteomics Combining Patch Clamp Electrophysiology and LC/MS/MS

Bio: John R. Yates is the Ernest W. Hahn Professor in the Departments of Molecular Medicine and Neurobiology at Scripps Research. His research interests include development of integrated methods for tandem mass spectrometry analysis of protein mixtures, bioinformatics using mass spectrometry data, and biological studies involving proteomics. He is the lead inventor of the SEQUEST software for correlating tandem mass spectrometry data to sequences in the database and developer of the shotgun proteomics technique for the analysis of protein mixtures. His laboratory has developed proteomic techniques to analyze protein complexes, posttranslational modifications, organelles and quantitative analysis of protein expression for the study of biology. He has received awards including the ASMS Biemann Medal, HUPO Achievement Award, Christian Anfinsen Award (Protein Society), Analytical Chemistry award (ACS), Ralph N. Adams Award, Thomson Medal (IMSF), John B. Fenn Award (ASMS), HUPO Discovery Award. He is currently the EIC at the Journal of Proteome Research

Yates

PETER M. YEHL, Executive Director, Small Molecule Analytical Chemistry, Small Molecule Pharmaceutical Sciences, Genentech Research and Early Development, South San Francisco, CA, USA

Next Generation Analytical Collaborations for Next Generation Therapeutics: Academic Partnering to Create a New and Essential Analytical Science Community

Bio: Peter Yehl has worked in the pharmaceutical industry for over 25 years and currently heads the Small Molecule Analytical Chemistry Department within Genentech Research and Early Development.  This group supports CMC discovery and development of drug substances and drug products from lead optimization through pivotal supply, covering all synthetic modalities, synthetic critical reagents and drug delivery systems.    Pete has lead and fostered numerous cross functional drug development teams to deliver complex therapeutic modalities and molecular systems for preclinical and clinical use.  Prior to Genentech, he worked at Merck Research Labs and PTC Therapeutics supporting classic small molecules, peptides and siRNA.  He is author or co-author of over 25 scientific papers.  He received his Ph.D. in Chemistry from the University of Massachusetts, Amherst and his B.Sc. in Engineering from Rochester Institute of Technology.

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YURY ZELECHONOK, President, SIELC, Wheeling, IL, USA

New Mode of Ions Separation

Bio: Dr. Yury Zelechonok was born in the USSR. He graduated in 1980 with a MS degree in chemical engineering from Ufa Petroleum Institute and received a Ph.D. in organic synthetic chemistry from the Soviet Academy of Science in 1985. He emigrated to the United States in 1989 when the Soviet Union started to fall apart. In the US he began to work as a postdoc with Professor Richard B. Silverman of Northwestern University. After 3 years he joined the pharmaceutical industry as a senior scientist in analytical discovery support at Pfizer/Monsanto/Searle. In 2003 Yury Zelechonok started the company SIELC Technologies with the idea to develop a new type of chromatography media: Mixed-mode, which was then successfully commercialized and is in common use now. Currently SIELC Technologies employs over 20 people and sells its products in all developed countries. Yury Zelechnok is the author of more than 30 patents related to different aspects of liquid chromatography.

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KELLY ZHANG, Director and Senior Principal Scientist, Small Molecule Analytical Chemistry, , Genentech, S. San Francisco, CA, USA

Characterization of Guide RNA for CRISPR Gene Editing

Bio: Kelly Zhang is a Director and Sr. Principal Scientist at Genentech in South San Francisco, California. She leads analytical chemistry R&D teams for drug substance and drug formulation, moving novel therapeutic molecules from research to clinical trials through collaboration with cross-disciplinary teams. She has published over 60 peer-reviewed papers in the field of analytical chemistry and pharmaceutical analysis. She is a Permanent Scientific Committee member of the international HPLC Conferences, and the international Pharmaceutical Biomedical Analysis Conferences. She was named three times on the Analytical Scientist power list: 2021 Top 100 list, 2019 Top 100 list, 2016 Top 50 most influential women.

ZHANG Kelly Powerlist

WENWAN ZHONG, Professor of Chemistry, Department of Chemistry, University of California-Riverside, Riverside, CA, USA

Coupling asymmetrical flow field flow fractionation (AF4) with capillary electrophoresis (CE) for analysis of extracellular vesicles

Bio: Dr. Wenwan Zhong obtained her B.S. from University of Science and Technology of China, and Ph.D. from Iowa State University. Her research is devoted to developing bioanalytical techniques for improvement of disease diagnosis and treatment. Her group employs diverse analytical techniques like flow-field flow fraction, capillary electrophoresis, microfluidics, optical spectroscopy, and mass spectrometry, and combines them with chemical tools such as synthetic receptors, functional nucleic acids, and nanomaterials, to enable the discovery, detection, and functional analysis of potential biomarkers. Her research has three current focuses: 1) Rapid processing and detection of cell-free biomarkers from biospecimens for clinical applications; 2) Development of chemical/biochemical probes to enable study of epigenetic factors like protein post-translational modification, and nucleic acid folding and modification, for better understanding of their biological functions; and 3) Study of the nano-bio interface to guide sustainable design and applications of nanomaterials.

Wenwan Zhong

ARNOLD ZOELDHEGYI, Molnár-Institute for Applied Chromatography, Berlin, GERMANY

Expediting the Characterization of COVID-19 Therapeutic mAbs using Innovative Ultra-short column Formats and Automated Chromatography-based Method Modeling

Bio: Arnold Zöldhegyi holds a master’s degree in Biochemical Engineering. During two years spent with Jenö Fekete’s HPLC group at University of Technology in Budapest, Arnold gained particular theoretical and practical skills in analytics. He joined the DryLab-Team in 2017 where he works as a graduate researcher. Together with Imre Molnár, Arnold contributed to several scientific papers and teaches Design Space Modeling for small and large molecules at various institutions internationally.

 

 

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